Journal: bioRxiv
Article Title: PLK4 inhibition as a strategy to enhance non-small cell lung cancer radiosensitivity
doi: 10.1101/2025.02.19.638860
Figure Lengend Snippet: Quantification and representative images of clonogenic survival analysis of H460 ( A ) and A549 ( B ) cells treated with CFI-400945 (10nM). Error bars represent standard error of the mean (SEM) for n=3 independent experiments analyzed by unpaired, two-tailed t-tests. Clonogenic survival analysis of H460 ( C ) or A549 ( D ) cells treated with vehicle (DMSO) or CFI-400945 (10nM) and the indicated dose of radiation. The results represent data from three independent experiments for each cell line. Data are represented as the mean ± SEM analyzed by two-way ANOVA testing. Clonogenic survival analysis of H460 ( E ) or A549 ( F ) cells treated with vehicle (DMSO) or centrinone (2.5 µM) and the indicated dose of radiation. The results represent data from three independent experiments for each cell line. Data are represented as the mean ± SEM analyzed by two-way ANOVA testing. G. Clonogenic survival analysis of MRC9 cells treated with vehicle (DMSO) or CFI-400945 (10nM) and the indicated dose of radiation. The results represent data from three independent experiments for each cell line. Data are represented as the mean ± SEM. H. Immunoblot of PLK4 expression in the indicated cell lines. Immunoblots are representative of three independent experiments.
Article Snippet: The human NSCLC cell lines H460 and A549, and human normal lung fibroblast cell line MRC9 were purchased from American Type Culture Collection (ATCC).
Techniques: Two Tailed Test, Western Blot, Expressing